Purification of hemoglobin

Details Purification of hemoglobin Purification of hemoglobin

C - Chemistry, Metallurgy

07

K

C07K 14/805 (2006.01) A61K 38/42 (2006.01) C07K 1/22 (2006.01)

Patent

CA 2176494

This invention relates to methods for the production of pure hemoglobin solutions, particularly pure hemoglobin solutions derived from expression of hemoglobin in recombinant systems, and compositions thereof. Methods are also disclosed for removal of contaminant hemoglobins using rapid heating to result in substantially protoporphyrin IX-free hemoglobin solutions, and for removal of high amounts of bacterial proteins and simultaneous purification of crude hemoglobin-oontaining starting materials to result in partially purified hemoglobin solutions. Methods for the further purification of partially purified hemoglobin solution and the removal of metal contamination in hemoglobin solutions, if required, are also disclosed.

L'invention porte sur des procédés de production de solutions d'hémoglobine pures et en particulier de solutions d'hémoglobine pure obtenues par expression d'hémoglobine dans des systèmes de recombinaison, et leur composition. Sont également présentés des procédés d'élimination par chauffage rapide d'hémoglobines contaminantes ce qui donne des solutions d'hémoglobine sensiblement exemptes de protoporphyrines IX, d'élimination de quantités élevées de protéines bactériennes, et de purification simultanée de produits de départ contenant de l'hémoglobine non purifiée, donnant des solutions d'hémoglobine partiellement purifiées. Sont en outre présentées des procédés de purification de solutions d'hémoglobine partielement purifiées et d'élimination, si nécessaire, de métaux contaminants de solutions d'hémoglobine.

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