G - Physics – 05 – D
Patent
G - Physics
05
D
G05D 23/19 (2006.01) B81B 1/00 (2006.01) B81B 7/00 (2006.01) C12M 1/36 (2006.01) C12M 1/38 (2006.01) C12M 1/40 (2006.01) G01N 27/10 (2006.01) G01N 27/447 (2006.01) H01L 35/28 (2006.01) H05B 3/00 (2006.01) H05B 3/60 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2300203
A method and device for tranporting and/or monitoring a fluid in a multi-port device used in a microfluidic system is provided. The multi-port device includes a substrate comprising a defined channel configuration. A first channel region having a first port and a second port for transporting fluid therebetween is defined in the substrate. A second channel region having a first port and a second port for applying an electric current for heating fluid or for monitoring a fluid parameter therebetween is also defined in the substrate. In some embodiments, the first channel intersects with the second channel. The heating or monitoring aspect of the invention can be used with a variety of biological reactions including polymerase chain reaction and ligase chain reaction.
La présente invention concerne un procédé et un dispositif de transport et/ou de surveillance d'un fluide dans un dispositif multiport utilisé dans un système microfluidique. Le dispositif multiport inclut un substrat comprenant une configuration définie du canal. Une première région du canal, définie dans le substrat, comporte un premier port et un second port permettant de transporter entre eux le fluide. Une seconde région du canal, également définie dans le substrat, comporte un premier port et un second port permettant d'appliquer un courant électrique servant à réchauffer le fluide ou à surveiller entre eux un paramètre du fluide. Selon certaines réalisations, le premier canal coupe le second canal. Les aspects chauffage ou surveillance de l'invention conviennent à une multitude de réactions biologiques, y compris l'amplification en chaîne par polymérase et la ligation répétitive d'oligonucléotides.
Chow Calvin Y. H.
Kopf-Sill Anne R.
Parce J. Wallace
Caliper Life Sciences Inc.
Caliper Technologies Corporation
Fetherstonhaugh & Co.
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