Probe biochips and methods for use thereof

Details Probe biochips and methods for use thereof Probe biochips and methods for use thereof

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2518367

The invention relates to the use of unmarked polynucleotide probes which can form hairpin loops, biochips comprising said probes and method for the use thereof. The invention further relates to methods for the production of said probes and biochips, more particularly the invention relates to the use of said unmarked probes and biochips for the manipulation and the analysis of polynucleotide sequences and optionally associated molecules. Furthermore, the invention relates to the methods for the preparation and use of said probes and biochips for the analysis of mutations, sequencing, detection of alternative splicing variants, analysis of gene expression, analysis of allelic disorders and loss of heterozygosity and the detection of all nucleic acids present in said organisms or residues from said organisms.

L'invention concerne les domaines d'utilisation des sondes polynucléotidiques non marquées, susceptibles de former des épingles à cheveux, les biopuces contenant de telles sondes ainsi que des méthodes permettant leur utilisation. La présente invention concerne également les méthodes permettant de créer de telles sondes ainsi que des biopuces. Plus particulièrement, l'invention concerne l'utilisation de telles sondes non marquées et biopuces pour la manipulation et l'analyse de séquences polynucléotidiques et éventuellement des molécules qui leurs sont associées. Cette invention concerne en outre les méthodes de préparation et d'utilisation de telles sondes et biopuces pour l'analyse de mutations, le séquençage, la détection de variants d'épissage alternatifs, l'analyse de l'expression de gènes, l'analyse des déséquilibres alléliques et des pertes d'heterozygotie, et la détection de tout acide nucléique présent dans les organismes ou résidus issus de ces organismes.

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