Multiwell in-vitro fertilization plate

Details Multiwell in-vitro fertilization plate Multiwell in-vitro fertilization plate

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) B01L 3/00 (2006.01) B65D 43/20 (2006.01) C12M 1/20 (2006.01)

Patent

CA 2123174

A plate assembly for useful for in vitro fertilization procuedures such as receiving ova and for forming, evaluating, holding, manipulating and culturing embryos includes a base with an apportioned top surface and a sidewall. The top surface has a plurality of open wells embedded therein for receiving ova to be cultured. The wells are substantially cylindrical and have substantially flat closed bottoms to facilitate microscopic examination of the well contents. The assembly includes a removable lid for covering the top surface of the base. The lid is preferably divided into sections comparable to the top surface portions of the base. Each lid section may be separately slidably moved between a closed position where the lid covers a comparable portion of the top surface and an open position where the top surface is uncovered. The lid has projections which interact with grooves in the base in a cam/cam follower relationship to allow slidable movement of the lid sections between the closed and the open positions while retaining the lid sections to the base. The inside surface of the lid and the base top surface each have elements which interact when the lid is in the closed position to form a tortuous path for gas interchange between the wells and the outside environment, thus substantially preventing microbial contamination of the wells when the lid is closed.

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