Rapid production of high titer virus

Details Rapid production of high titer virus Rapid production of high titer virus

C - Chemistry, Metallurgy

12

N

C12N 15/867 (2006.01) A61K 48/00 (2006.01) C07K 14/15 (2006.01) C12N 7/00 (2006.01) C12N 15/09 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2622074

The invention includes methods of producing viral particles which include introducing into avian cells a nucleotide sequence encoding a replication deficient retroviral vector and introducing into the avian cells nucleotide sequences encoding products required for replication of the replication deficient retroviral vector under the control of a promoter that is functional in the avian cell line, and harvesting the viral particles.

L'invention porte sur des procédés de production de particules virales qui consistent: à introduire dans des cellules aviaires une séquence nucléotidique codant un vecteur rétroviral à réplication déficiente; à introduire dans les séquences nucléotidiques des cellules aviaires les produits de codage requis pour la réplication du vecteur rétroviral à réplication déficiente sous le contrôle d'un promoteur qui est fonctionnel dans la lignée de cellules aviaires; et à récolter les particules virales.

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