C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/22 (2006.01) C12N 5/06 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2278847
The invention is directed to methods for the production of selected populations of lymphocytes. Lymphocytes produced can be isolated and purified using well known and established procedures to provide a consistent lymphocyte source which one of ordinary skill in the art can modify to provide an appropriate type or an optimal level of a desired lymphocyte. The availability of such cell populations allows not only for the complete reconstitution of the depleted, defective or missing lymphocyte population in a patient, but also provides the flexibility of having sufficient cells to permit multiple or cyclic treatments. These methods for expanding target cell populations are broadly applicable to the selective expansion of several types of lymphocytes and are demonstrated to maintain phenotype as well as antigen specificity.
L'invention concerne un procédé permettant de produire des populations sélectionnées de lymphocytes. Les lymphocytes produits peuvent être isolés et purifiés par des techniques connues et établies, de façon à constituer une source stable de lymphocytes, qu'une personne versée en la matière peut modifier afin d'obtenir un type approprié ou un taux optimal de lymphocytes désirés. Ces populations cellulaires permettent de reconstituer totalement la population épuisée, déficiente ou manquante chez un patient, et, de plus, elles sont suffisamment souples pour donner la quantité de cellules nécessaire à des traitements multiples ou cycliques. Ces procédés permettant d'augmenter les populations cellulaires peuvent être largement utilisés pour accroître sélectivement la quantité de plusieurs types de lymphocytes, sans modifier, comme cela a été prouvé, leur phénotype et leur spécificité antigénique.
Bell David
Wong Truman
Bereskin & Parr
Hemosol Inc.
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