Method of activating protein

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) C07K 1/00 (2006.01) C12N 1/00 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2468971

A method of producing a protein having free cysteine with the use of a serum- free medium, characterized in that the protein is produced in the activated state; a method of producing a protein by culturing in a serum-free medium in accordance with the above method; a method of activating a protein having free cysteine which has been produced in the inactivated state; and a protein obtained by any of the above methods. This protein shows physicochemical or biological properties comparable to a protein obtained by using a serum medium.

L'invention concerne un procédé de production de protéine à cystéines libres au moyen d'un milieu exempt de sérum, ce procédé se caractérisant en ce que la protéine est produite à l'état activé. L'invention concerne également un procédé de production de protéine par culture de protéine dans un milieu exempt de sérum, selon le procédé décrit ci-dessus. L'invention concerne encore un procédé d'activation de protéine à cystéines libres, produite à l'état inactivé. L'invention concerne enfin une protéine obtenue par la mise en oeuvre d'un des procédés décrits ci-dessus. Cette protéine présente des propriétés physico-chimiques ou biologiques comparables à celles d'une protéine obtenue au moyen d'un milieu sérique.

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