Polynucleotide matrix-based method of identifying...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01J 19/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2370255

Method of identifying microbial organisms wherein a biological sample containing nucleic acids is hybridized with a collection of polynucleotide probes, each probe having binding specificity for the ribosomal nucleic acids of at least one microbe. The collection of probes isorganized into a series of "addresses" that provide information about the presence or absence of one or more polynucleotide sequences in the biological sample. Probes in the matrix are selected to distinguish between organisms that differ from each other by a known phylogenetic relationship. Advantageously, the invented method can detect and resolve the identities of microorganisms that are present in a mixed culture. The system is particularly suited to automated analysis.

Cette invention concerne une méthode d'identification de micro-organismes. Selon cette méthode, on hybride un échantillon renfermant des acides nucléiques avec une collection de sondes polynucléotidiques qui présente chacune une spécificité de liaison pour les acides nucléiques ribosomiques d'au moins un microbe. La collection de sondes est agencée selon un série <= d'adresses >= qui renseignent sur la présence ou l'absence d'une ou de plusieurs séquences polynucléotidiques dans l'échantillon biologique. On sélectionne les sondes de la matrices de manière à pouvoir distinguer les uns des autres des micro-organismes qui diffèrent par une relation phylogénétique connue. La méthode selon l'invention permet avantageusement de détecter et de résoudre les identités de micro-organismes présents dans une culture mixte. Ce système convient pour l'analyse automatisée.

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