Root-preferred promoters and their use

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2348665

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Knox1, a Kn1-like homeobox gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

La présente invention concerne des compositions et des procédés permettant de réguler l'expression de séquences nucléotidiques hétérologues dans une plante. Les compositions contiennent une nouvelle séquence nucléotidique destinée à un promoteur à préférence radicalaire pour le codage du gène Knox1, un gène d'homéoboîte du type Kn-1t des séquences isolées à partir de ce dernier. On décrit un procédé d'expression d'une séquence nucléotidique hétérologue dans une plante à l'aide des séquences de promoteur de l'invention. Le procédé consiste à incorporer de manière stable dans le génome d'une cellule de plante une séquence nucléotidique liée de manière fonctionnelle au promoteur à préférence radicalaire de l'invention et à régénérer une plante transformée de manière stable qui exprime la séquence nucléotidique.

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