Process for the separation of glycosylated and...

Details Process for the separation of glycosylated and... Process for the separation of glycosylated and...

C - Chemistry, Metallurgy

07

K

C07K 1/16 (2006.01) C07K 1/22 (2006.01) C07K 14/76 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2292889

A process for the partial or complete separation of glycosylated and nonglycosylated proteins is described, in which a) a triazine dye immobilized on a matrix is incubated with a mixture of glycosylated and nonglycosylated proteins, b) the matrix is then washed to remove the unbound proteins, and c) the proteins are eluted by means of a stepwise or continuous increase in the ionic strength or in the pH, nonglycosylated proteins and proteins having an increasing degree of glycosylation being collected separately from one another in the eluate fractions obtained. By means of the use of this process it is possible, for example, to prepare a human albumin which is free of glycoside bonds and is expressed in yeast cells.

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