Method for potentiating tissue plasminogen activators

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) C07K 16/40 (2006.01) C12N 9/72 (2006.01) C12Q 1/56 (2006.01) G01N 33/573 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2221383

A method for potentiating the activity of tissue plasminogen activator proteins by adding certain amounts of beta-lactoglobulin A is claimed. In particular, the invention includes an enhanced solid-phase immunoassay for measuring tissue plasminogen activator (nt-PA) activity in a mammalian serum sample with sufficient sensitivity to detect neutralizing activity in samples containing µg/ml levels of total nt-PA antibody. Neutralizing activity can be easily detected in serum dilutions containing as little as 0.038 to 0.48 µg of total nt-PA antibody in a 20 µl sample. The assay has a sensitivity of 1 pM of nt-PA (67 pg/ml) in 5 hr.

Cette invention concerne un procédé de potentialisation de l'activité de protéines d'activateurs plasminogènes de tissus par l'addition de certaines quantités de bêta-lactoglobuline A. Cette invention concerne en particulier un dosage immunologique amélioré en phase solide destiné à mesurer l'activité de l'activateur plasminogène de tissus (nt-PA) dans un échantillon de sérum d'un mammifère, lequel dosage possède une sensibilité suffisante pour détecter une activité neutralisante dans des échantillons contenant des concentrations en µg/ml d'anticorps nt-PA total. Une activité neutralisante peut facilement être détectée dans des dilutions de sérum contenant une concentration faible de 0,038 à 0,48 µg d'anticorps nt-PA total dans un échantillon de 20 µl. Ce dosage possède une sensibilité de 1 pM de nt-PA (67 pg/ml) sur une période de 5 heures.

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