Fluorescence-based high throughput screening assays for...

Details Fluorescence-based high throughput screening assays for... Fluorescence-based high throughput screening assays for...

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) C12Q 1/42 (2006.01) G01N 33/573 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2306686

The invention relates to novel fluorescence-based assays for protein kinases and phosphatases which can be used in high throughput screening. The methods of the invention utilize a competitive immunoassay to determine the amount of substrate that is phosphorylated or dephosphorylated during the course of a kinase or phosphatase reaction to yield a product, as well as the phosphorylating or dephosphorylating activity of a kinase or phosphatase.

L'invention concerne des essais à base de fluorescence pour protéines kinases et phosphatases, qui rendent possible un criblage à haut rendement. Les procédés décrits reposent sur le principe du dosage immunologique par compétition, qui permet de déterminer la quantité de substrat phosphorylée ou déphosphorylée en cours de réaction kinase ou phosphatase de manière à livrer un produit, et de déterminer aussi l'activité de phosphorylation ou de déphosphorylation d'une kinase ou d'une phosphatase.

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