Process for producing aspartase and process for producing...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/88 (2006.01) C12P 13/20 (2006.01)

Patent

CA 2200420

Disclosed are a process for producing aspartase which comprises culturing a microorganism belonging to the genus Escherichia being capable of producing aspartase in a medium until aspartase is produced and accumulated in the culture, and recovering aspartase therefrom, wherein dissolved oxygen concentration of the medium is in the range of 0 to 1 ppm on the stage that microbial growth is middle and/or late logarithmic growth phase; and a process for producing L-aspartic acid which comprises converting fumaric acid and ammonia into L-aspartic acid in an aqueous medium in the presence of an enzyme source, wherein said enzyme source is a culture produced in accordance with said aspartase-producing process, cells isolated from the culture, or processed cells thereof.

On divulgue un procédé pour produire de l'aspartase qui comprend la culture d'un microorganisme appartenant au genre Escherichia capable de produire de l'aspartase dans un milieu jusqu'à ce que l'aspartase s'accumule dans le milieu de culture, et pour la récupération de l'aspartase du milieu, où la concentration d'oxygène dissous du milieu varie de 0 à 1 ppm au milieu et/ou à la fin de la phase de croissance exponentielle du microorganisme et un procédé pour produire de l'acide L-aspartique qui comprend la conversion de l'acide fumarique et d'ammoniac en acide L-aspartique dans un milieu aqueux en présence d'une source d'enzyme, où ladite source d'enzyme est une culture produite conformément audit procédé de production d'aspartase, les cellules isolées de la culture ou les cellules transformées de la culture.

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