Process for constructing a mutant utilizing the igh 3' enhancer

Details Process for constructing a mutant utilizing the igh 3' enhancer Process for constructing a mutant utilizing the igh 3' enhancer

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) A01K 67/00 (2006.01) C07K 16/18 (2006.01) C12N 5/16 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2451608

The invention provides a production process for a high-frequent random mutant of an object exogenous gene, wherein a recombinant expression vector in which a eukaryote promoter, an exogenous DNA sequence, an intron enhancer and 3'HS3/4 enhancer are linked is introduced and expressed in an animal cell.

La présente invention concerne procédé permettant de construire un mutant aléatoire haute-fréquence d'un gène étranger à une cible. Ce procédé implique de transférer dans des cellules animales un vecteur d'expression recombinant dans lequel sont réunis par ligation un promoteur eucaryote, une séquence d'ADN étranger, un renforceur d'intron et un renforceur 3'HS3/4, et de faire suivre par l'expression.

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