Process for producing magnetically responsive polymer...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) A61K 47/48 (2006.01) B03C 1/00 (2006.01) B03C 1/01 (2006.01) C07K 1/22 (2006.01) C07K 17/00 (2006.01) C12N 11/08 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01) H01F 1/36 (2006.01) H01F 1/37 (2006.01)

Patent

CA 2087976

This invention provides a novel process of producing magnetically responsive polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used as core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, ~~~ separation and other medical, diagnostic, and industrial applications.

Nouveau procédé de production de particules polymères à sensibilité magnétique comportant des particules polymères centrales enrobées de manière homogène d'une couche polymère contenant un oxyde métallique à sensibilité magnétique. On peut utiliser comme particules centrales une grande diversité de particules polymères ayant des dimensions allant de 1 à 100 microns, et on peut les transformer en particules polymères à sensibilité magnétique. En outre, on peut enrober la surface de ces dernières d'une couche supplémentaire de polymère fonctionnalisé. Lesdites particules polymères à sensibilité magnétique peuvent servir au couplage passif ou covalent de matières biologiques telles que les antigènes, les anticorps, et les enzymes, ou à l'hybridation ADN/ARN, et peuvent servir de phase solide pour divers types de dosages immunologiques, de dosages de sondes d'hybridation d'ADN/ARN, de purification d'affinité, et de séparation cellulaire, ainsi que pour d'autres applications médicales, diagnostiques et industrielles.

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