Preparation method

G - Physics – 01 – N

Patent

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Details

G01N 1/34 (2006.01) A61K 35/14 (2006.01) C12N 5/00 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2665205

A method of storing and purifying cells from a whole blood sample, comprising storing the sample before and/or after purification of cells for at least 6 hours, and purifying a population of lymphocytes and antigen presenting cells for use in a cell-mediated immunoassay (CMI assay), by a method incorporating a positive or negative affinity selection step.

L'invention concerne un procédé de préparation destiné à stocker et à purifier des cellules à partir d'un échantillon sanguin dans son intégralité, à stocker l'échantillon avant et/ou après purification des cellules durant au moins 6 heures, et à purifier une population de lymphocytes et d'antigènes présentant des cellules utilisées dans un dosage immunologique induit par des cellules (dosage CMI), au moyen d'un procédé contenant une étape de sélection d'affinité positive ou négative.

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