Method of extracting virus from cell culture

Details Method of extracting virus from cell culture Method of extracting virus from cell culture

C - Chemistry, Metallurgy

12

N

C12N 7/02 (2006.01) A61K 35/76 (2006.01) A61P 35/00 (2006.01) C12N 7/00 (2006.01)

Patent

CA 2437962

The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25 ~C or 37 ~C to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.

La présente invention concerne une méthode d'extraction d'un virus, et notamment d'un réovirus, à partir d'une culture cellulaire. Le virus infectieux peut être extrait de cette culture au moyen d'un détergent à une température adaptée telle que 25 ·C ou 37 ·C en vue d'obtenir des titres viraux élevés. La présente invention permet d'utiliser des détergents ioniques et non ioniques.

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