Expression vectors based on modified ribosomal protein...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2729254

The present invention relates to expression vector comprising (a) a promoter region comprising a non-inducible constitutively active ribosomal protein gene promoter, (b) an operably linked reporter or gene sequence, and (c) a 3' untranslated region (3' UTR), which are suitable means for an selective assessment of post- transcriptional regulation, post-transcriptional con-trol elements and factors as well as for identifying compounds that effect post-transcription. The present invention furthermore re-lates to arrays, expression vector libraries and cell lines containing the expression vector(s). The present invention furthermore re-lates to a method and kit for identifying compounds that affect post- transcriptional regulation of reporter(s) or gene(s), that utilize the expression vector(s).

La présente invention concerne un vecteur dexpression comprenant (a) une région de promoteur comprenant un promoteur de gène de protéine ribosomique constitutivement actif, non inductible, (b) un rapporteur ou séquence génique fonctionnellement lié, et (c) une région non traduite 3' (3'-UTR), qui sont des moyens adaptés pour une évaluation sélective de la régulation post-transcriptionnelle, des éléments et des facteurs de contrôle post-transcriptionnel ainsi que pour identifier des composés ayant un effet post-transcription. La présente invention concerne en outre des puces, des bibliothèques de vecteurs dexpression et des lignées cellulaires contenant le(s) vecteur(s) dexpression. La présente invention concerne en outre un procédé et un kit pour identifier des composés qui affectent la régulation post-transcriptionnelle de rapporteur(s) ou de gène(s), qui utilisent le(s) vecteur(s) dexpression.

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