Methods and devices for concentration and purification of...

Details Methods and devices for concentration and purification of... Methods and devices for concentration and purification of...

H - Electricity

01

J

H01J 49/04 (2006.01)

Patent

CA 2541536

Analytical methods and devices are disclosed for separating low abundance analytes by electrophoretically driving the analytes through a sieving matrix to first remove high molecular weight species. Subsequently the remaining low abundance analytes are electrophoretically focused onto a capture membrane where the analytes become bound within a small capture site. After this step the capture membrane may be allowed to dry and then attached to a conductive MALDI sample plate.

L'invention concerne des procédés analytiques et des dispositifs destinés à séparer des analytes peu abondants et permettant d'entraîner les analytes par voie électrophorétique à travers une matrice de tamisage en vue d'enlever d'abord les espèces de poids moléculaire élevé. Ensuite, les analytes peu abondants restants sont concentrés par voie électrophorétique sur une membrane de capture, les analytes se liant à l'intérieur d'un site de capture restreint. Après cette étape, la membrane de capture peut sécher et être fixée à une plaque d'échantillon MALDI conductrice.

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