Method to detect candida species

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/04 (2006.01) C12N 15/31 (2006.01) C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2715575

DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from(i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis,Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseriaand Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifyingin a test sample antibiotic resistance genes selected from the group consisting of bla tem, bla rob, bla shv, bla oxa, blaZ, aadB, aacC1, aacC2,aacC3, aacA4, aac6'-IIa, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6'-aph(2"), aad(6'), vat, vga, msrA, sul and int are alsodisclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis.These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and/or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and/or genera, and/or (iii) the detection, identification and quantificationof the above-mentioned antibiotic resistance genes are also claimed.

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