Recombinant production of carotenoids, particularly astaxanthin

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

IPC codes

C12N 15/09 (2006.01) C12N 1/14 (2006.01) C12N 9/02 (2006.01) C12N 9/08 (2006.01) C12N 15/53 (2006.01) C12P 23/00 (2006.01) C12Q 1/68 (2006.01)

Type

Patent

Patent number

CA 2324625

Description

The present invention is directed to a process for producing carotenoids comprising cultivating a recombinant organism having a gene for one or more active oxygen species-quenching factor(s) that is substantially disrupted with a disruption cassette specific to the gene, and recovering carotenoids from the culture, as well as to genetic materials useful in the said process, such as a recombinant organism producible of carotenoids, a disruption cassette, a recombinant DNA sequence, a recombinant DNA fragment; and a polynucleotide.

La présente invention porte sur un procédé permettant de produire des caroténoïdes. Le procédé comprend la culture d'un organisme recombinant présentant le gène d'un ou de plusieurs facteurs de désactivation des espèces oxygénées activées, gène subissant une disruption en présence d'une cassette de disruption spécifique, et la récupération des caroténoïdes dans le milieu de culture. Le procédé porte aussi sur le matériel génétique utile audit procédé, comme l'organisme recombinant produisant les caroténoïdes, la cassette de disruption, une séquence d'ADN recombinant, un fragment d'ADN recombinant et un polynucléotide.

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