A method for preparing a thromboplastin reagent from...

Details A method for preparing a thromboplastin reagent from... A method for preparing a thromboplastin reagent from...

C - Chemistry, Metallurgy

12

Q

C12Q 1/56 (2006.01) C07K 14/745 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2080428

A method for preparing a thromboplastin reagent from cultured human cells comprising the steps of washing the cells with isotonic aqueous salt solution, lysing the cells by hypotonic shock, and resuspending the cell lysate in a diluent compatible with a one- stage prothrombin time determination to reach a concentration of about 0.5 to 3.0 mg/mL thromboplastin, whereby said cell lysate thromboplastin suspension produces a clot in a one stage prothrombin time test in about 10 to 15 seconds, with a mean normal prothrombin time of about 11-13 seconds, when added to citrated normal plasma in a volume ratio of about 2:1, such thromboplastin being stable for at least 8 hours at 37°C and for at least 5 days at 4°C.

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