Methods for generating genetically altered...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C07K 16/00 (2006.01) C07K 16/42 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12P 21/06 (2006.01) C12Q 1/68 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2428214

Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production.

Des allèles dominants négatifs de gènes humains de réparation de mésappariements peuvent être utilisés pour générer des cellules et des organismes hypermutables. En introduisant ces gènes dans des cellules et des animaux transgéniques, on peut produire de nouvelles lignées cellulaires et de nouvelles variétés animales présentant de nouvelles propriétés utiles avec une plus grande efficacité qu'en se basant sur le taux naturel de mutation. Ces procédés permettent de générer une diversité génétique à l'intérieur de gènes des immunoglobulines visant un antigène spécifique pour produire des anticorps modifiés présentant une activité biochimique accrue. Ces procédés permettent en outre de générer des cellules produisant des anticorps avec un niveau accru de production d'anticorps.

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