Ligand detection method

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2437043

There is disclosed a sensitive method for specific detection of ligands comprising the steps of (a) contacting the sample with reagents capable of forming a reagent complex, which reagent complex comprises a receptor capable of specifically binding to said ligand and a nucleic acid molecule; and (b) detecting any complexes formed by binding of the receptor part of said reagent complex to ligand present in the sample by specifically detecting the presence of the nucleic acid molecule by amplifying a region of the nucleic acid and simultaneously detecting products of the amplification reaction in real-time.

L'invention concerne un procédé sensible destiné à la détection spécifique de ligands consistant à mettre en contact l'échantillon avec des réactifs capable de former un complexe réactif, ce complexe réactif comprenant un récepteur capable de se lier de manière spécifique à ce ligand et une molécule d'acide nucléique, et (b) à détecter tous les complexes formés par liaison de la partie récepteur du complexe réactif au ligand présent dans l'échantillon par détection spécifique de la présence de la molécule d'acide nucléique par amplification d'une zone de l'acide nucléique et à détecter simultanément des produits de la réaction d'amplification en temps réel.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Ligand detection method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ligand detection method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ligand detection method will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1501205

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.