General means of labeling protein by using wheat embryo...

Details General means of labeling protein by using wheat embryo... General means of labeling protein by using wheat embryo...

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2416684

A process for producing a selemethionine-labeled protein characterized by using a wheat embryo cell-free protein synthesis system, converting methionine, which is contained in a cell extract for synthesizing a cell-free protein obtained by completely eliminating albumen contained in a wheat embryo extract from the embryo extract, into selemethionine, and then synthesizing a cell-free protein with the use of the protein synthesis reaction mixture containing selemethionine as a substitute for methionine under batch conditions or dialysis conditions; and the protein thus produced. Moreover, a process for producing a deuterium-labeled protein by using the same procedure and the protein thus produced are provided.

L'invention concerne un procédé de production d'une protéine marquée à la séléméthionine consistant à utiliser un système de synthèse de protéine acellulaire d'embryon de blé, à convertir en séléméthionine la méthionine contenue dans un extrait cellulaire destiné à synthétiser une protéine acellulaire obtenue par suppression totale de l'albumen contenu dans un embryon de blé issu cet extrait cellulaire, puis à synthétiser une protéine acellulaire au moyen d'un mélange réactionnel de synthèse de protéine contenant de la séléméthionine comme produit de substitution pour la méthionine dans des conditions de traitement discontinu ou de dialyse. L'invention concerne également la protéine ainsi produite. Elle se rapporte en outre à un procédé destiné à produire une protéine marquée au deutérium au moyen de cette même opération, ainsi qu'à la protéine ainsi produite.

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