Proteases fused with variants of streptomyces subtilisin...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 14/47 (2006.01) C07K 14/81 (2006.01) C07K 19/00 (2006.01) C12N 9/54 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2332177

The present invention relates to fusion proteins wherein the fusion protein comprises a protease part; and a variant part, wherein the variant part has a modified amino acid sequence of a parent amino acid sequence, wherein the modified amino acid sequence comprises an amino acid substitution at position 63 corresponding to SSI, and wherein the parent amino acid sequence is selected from the group consisting of SSI, SSI-like inhibitors, variants of SSI, and variants of SSI-like inhibitors. Such fusion proteins are useful in cleaning compositions and personal care compositions. The present invention also relates to cleaning compositions and personal care compositions comprising the present fusion proteins, as well as DNA encoding the fusion proteins.

Protéines hybrides qui comportent une partie protéase et une partie variant, la partie variant possédant une séquence d'acides aminés modifiée d'une séquence d'acides aminés mère. La séquence d'acides aminés modifiée comporte une substitution d'acide aminé à la position 63 correspondant à l'inhibiteur de subtilisine de Streptomyces (SSI). La séquence d'acides aminés mère est choisie dans le groupe constitué par SSI, des inhibiteurs de type SSI, des variants de SSI et des variants d'inhibiteurs de type SSI. Ces protéines hybrides sont utiles dans des compositions de nettoyage et des compositions d'hygiène personnelle. La présente invention concerne également des compositions de nettoyage et des compositions d'hygiène personnelle comportant lesdites protéines hybrides, ainsi que de l'ADN codant ces protéines.

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