C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12P 21/08 (2006.01)
Patent
CA 2503315
The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval, in which the cells must acquire the ability to survive in a protein-free medium. There is a specific critical protein concentration interval for each cell line. The invention also relates to mammal cell lines which grow in a stable manner in a medium free of serum and proteins over at least 40 generations. The invention further relates to clones which express anti-CD6 T1hT and EGF hR3 antireceptor humanised antibodies and the anti-CD3 T3Q chimeric antibody as well as fragments of said antibodies.
La présente invention concerne une méthode d'obtention de cellules de mammifères conçues pour se développer dans un milieu exempt de sérum et de protéines, comprenant un processus en deux étapes d'adaptation dans lesquel il existe un intervalle critique de concentration de protéine dans lequel les cellules doivent être capables de survivre dans un milieu exempt de protéines. L'intervalle critique de concentration de la protéine est spécifique à chaque lignée cellulaire. La présente invention concerne également des lignées cellulaires de mammifères qui se développent de manière stable dans un milieu exempt de sérum et de protéines pendant au moins 40 générations. La présente invention concerne également des clones qui expriment les anticorps humanisés anti-récepteur de l'EGF hR3 et anti-CD6 T1hT et l'anticorps chimérique anti-CD3 T3Q, en plus des fragments desdits anticorps.
Boggiano Ayo Tammy
Castillo Vitlloch Adolfo
Perez Rodriguez Rolando
Rojas Del Calvo Luis
Vitores Sarazola Svieta
Centro de Inmunologia Molecular
Heenan Blaikie Llp
LandOfFree
Method of obtaining cell lines in a protein-free medium and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of obtaining cell lines in a protein-free medium and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of obtaining cell lines in a protein-free medium and... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1503020