Production of dicarboxylic acids by improved mutant strains...

Details Production of dicarboxylic acids by improved mutant strains... Production of dicarboxylic acids by improved mutant strains...

C - Chemistry, Metallurgy

12

P

C12P 7/40 (2006.01)

Patent

CA 2590795

The invention concerns a method for producing dicarboxylic acids (DCA) with long hydrocarbon chains, also called diacids, which consists in culturing a mutant strain of Yarrowia lipolytica obtained by mutagenesis directed and more particularly disrupted at least for the POX2, POX3, POX4 and POX5 genes encoding acyl-CoA oxydase, in a medium consisting essentially of an energetic substrate including at least one carbon source and one nitrogen source and in subjecting said strain to a bioconversion substrate selected among n-alkanes of at least 10 carbon atoms, fatty acids of at least 10 carbon atoms, their alkyl esters and natural oils.

L'invention concerne un procédé de production d'acides dicarboxyliques (DCA) à longues chaînes hydrocarbonées, également appelés diacides, dans lequel on met en culture une souche mutante de Yarrowia lipolytica obtenue par mutagénèse dirigée et plus particulièrement disruptée au moins pour les gènes POX2, POX3, POX4 et POX5 codant pour Pacyl-CoA oxydase, dans un milieu constitué essentiellement d'un substrat énergétique qui comprend au moins une source de carbone et une source d'azote et on soumet ladite souche à un substrat de bioconversion choisi parmi les n-alcanes d'au moins 10 atomes de carbone, les acides gras d'au moins 10 atomes de carbone, leurs esters d'alkyle et les huiles naturelles.

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