Modified type a dna polymerases

C - Chemistry – Metallurgy – 12 – N

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C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2742594

The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

La présente invention porte sur des ADN polymérases améliorées, en particulier sur des ADN polymérases de type A, qui peuvent être mieux adaptées à des applications dans des technologies d'ADN recombinant. Entre autres choses, la présente invention porte sur des ADN polymérases modifiées issues d'expériences d'évolution dirigée conçues pour sélectionner des mutations qui procurent des phénotypes avantageux dans des conditions utilisées dans des applications industrielles ou de recherche.

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