Immunoassay for the simultaneous immunochemical...

G - Physics – 01 – N

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G01N 33/543 (2006.01)

Patent

CA 2640835

The invention relates to an immunoassay for the simultaneous immunochemical determination of an analyte (antigen) and a treatment antibody targeting the analyte in samples (recovery immunoassay). The inventive immunoassay comprises an optionally marked catcher antibody bound to a surface, a marked treatment antibody, or a marked antibody which has the same binding epitope as the treatment body, an antigen that binds to the catcher antibody and the treatment antibody in the region of various epitopes, and in this way forms an immunochemical sandwich, a solution of the unmarked treatment antibody of a known concentration, and an antigen solution to be added to the samples. The invention can be applied to the fields of medical diagnosis, treatment control and pharmacological research.

L'invention concerne un dosage immunologique pour analyser simultanément par immunochimie un analyte (antigène) et un anticorps thérapeutique dirigé contre cet analyte dans des échantillons (dosage immunologique faisant intervenir la récupération). Ce dosage immunologique comprend un anticorps de capture éventuellement marqué lié à une surface ; un anticorps thérapeutique marqué, ou un anticorps marqué présentant le même épitope de liaison que l'anticorps thérapeutique ; un antigène qui se lie à l'anticorps de capture ou à l'anticorps thérapeutique au niveau de différents épitopes et forme ainsi un sandwich immunochimique ; une solution de l'anticorps thérapeutique non marqué de concentration connue ; une solution d'antigène à ajouter aux échantillons. Cette invention peut être mise en application dans les domaines du diagnostic médical, de la surveillance thérapeutique et de la recherche pharmacologique.

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