G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/15 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2352614
It is an object of the present invention to provide inexpensive materials and methods for screening immunotherapeutic agents exhibiting antitumor and/or anticancer activity. The present invention provides a method for determining in vitro a material having an LAK activity--enhancing effect suitable for a subject, comprising the steps of: (a) collecting peripheral blood from the subject to prepare lymphocyte fractions, (b) preparing an LAK-induced sample and a control sample by adding or not a screening material of the present invention to said lymphocyte fractions, and (c) measuring the LAK activities of said induced sample and said control sample and comparing the results to determine the in vitro LAK activity-enhancing effect of the screening material for said subject.
L'invention concerne une substance bon marché destinée à cribler un agent immuno-thérapeutique ayant une activité anti-tumorale et/ou anti-cancéreuse. Elle concerne une méthode qui permet de déterminer in vitro une substance ayant un effet approprié de potentialisation de l'activité LAK chez un sujet. La méthode consiste à: a) recueillir le sang périphérique du sujet et préparer une fraction lymphocytaire de ce sang; b) préparer, d'une part, un prélèvement d'induction de LAK par adjonction de la substance de criblage à la fraction lymphocytaire, d'autre part, un prélèvement de contrôle exempt de substance de criblage; et c) mesurer l'activité LAK dans le prélèvement d'induction et l'activité LAK dans le prélèvement de contrôle, et comparer ces activités afin de déterminer l'effet de potentialisation de l'activité LAK de la substance de criblage in vitro sur ledit sujet.
Asano Kenji
Matsuda Yukiko
Tajima Yutaka
Kobayashi Pharmaceutical Co. Ltd.
Nagaoka Hitoshi
Norton Rose Canada S.e.n.c.r.l.,s.r.l./llp
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