Method of diagnosing pituitary dependent growth hormone...

G - Physics – 01 – N

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G01N 33/74 (2006.01)

Patent

CA 2216260

A diagnostic protocol and kit for evaluating pituitary growth hormone (GH) secretory capability as a means to identify the etiology of GH deficiency in children and adults is disclosed. The inventive protocol includes measuring GH secretion, provoked by independent, sequential administration of the xenobiotic GH releasing hexapeptide (GHRP-6) or any of its peptidyl or non-peptidyl synthetic analogues (GHRX) that release GH by the same cellular mechanism as GHRP-6 followed by the naturally occurring GH releasing hormone or its analogues (GHRH) to children and adults afflicted with disorders related to GH deficiency. After evaluating the effects of independent, sequential administration of the GH secretagogues upon changes in blood GH concentrations, the effect of the co-administered GH secretagogues on blood GH concentrations in the child or adult subject is then measured.

Méthode et trousse de diagnostic pour évaluer la capacité de sécrétion d'hormone de croissance (GH) hypophysaire, comme moyen pour déterminer l'étiologie de la carence en GH chez les enfants et les adultes. La méthode consiste à mesurer la sécrétion de GH, provoquée par l'administration indépendante et séquentielle d'hexapeptide xénobiotique (GHRP-6), libérant GH, ou de l'un quelconque de ses analogues synthétiques peptidyliques ou non peptidyliques (GHRX), libérant GH par le même mécanisme cellulaire que GHRP-6, suivie de l'hormone naturelle libérant GH, ou de l'un de ses analogues (GHRH), à des enfants ou à des adultes souffrant de troubles liés à la carence en GH. Après évaluation des effets de l'administration indépendante et séquentielle de sécrétagogues de GH sur les variations des concentrations de GH dans le sang, on a mesuré l'effet de la co-administration de sécrétagogues de GH sur les concentrations de GH dans le sang chez des enfants et des adultes.

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