The lupac bifunctional marker and its use in protein production

Details The lupac bifunctional marker and its use in protein production The lupac bifunctional marker and its use in protein production

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A61K 38/00 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 15/53 (2006.01) C12N 15/54 (2006.01) C12N 15/65 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/66 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2593429

This invention relates to industrial production of proteins. More specifically, the invention relates to the Lupac surrogate marker, which corresponds to a fusion between luciferase and the puromycin N-acetyl transferase. The invention further relates to the use of Lupac for screening cells for high expression of a protein of interest.

Cette invention porte sur la production industrielle de protéines et, plus spécifiquement, sur un critère de substitution Lupac qui correspond à une fusion entre la luciférase et la puromycin N-acétyl transférase. L'invention porte également sur l'utilisation de Lupac dans la recherche systématique de cellules pour une expression élevée d'une protéine considérée.

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