G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/569 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2495138
Clinical samples can be analyzed using microparticles as a platform to determine the serodiagnosis of a viral infection from two candidate viral infections of the same viral group in a clinical sample. Various techniques can be used to determine serodiagnosis of viral infections; however, detection of a viral infection from two candidate viral infections of the same viral group in a clinical sample represents a significant advance. For example, serodiagnosis of a viral infection from two candidate viruses of the same viral group can be determined via a pooled population of subsets of microparticles, with the particles in the pooled population having a bound viral group-reactive antibody and the particles in each subset having at least one characteristic classification parameter that distinguishes one subset from those of another subset. Viral antigens of antibodies of interest in the same viral-class as the viral group-reactive antibody can be bound to the viral group-reactive antibody on the microparticles, and subsequently exposed to a clinical sample. Viral antibodies in the clinical sample can bind with the antigens and a labeled reactant can be used to label the viral antibodies bound to the antigens. Multiplexed flow analysis and computer-implemented methods of analyzing the data produced from flow analysis can then be used to determine the presence or absence of antibodies of interest in the clinical sample, thereby diagnosing a viral infection from two candidate viral infections in a single assay.
Biggerstaff Bradley J.
Johnson Alison Jane
Smart & Biggar
The Government Of The United States Of America As Represented By Centers For Disea
LandOfFree
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