Method for the affinity isolation of newly synthesized rna

Details Method for the affinity isolation of newly synthesized rna Method for the affinity isolation of newly synthesized rna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2359814

Methods are provided for the isolation of newly synthesized mRNA. Such methods involve the incorporation of biotinylated rNTP analogues into cellular mRNA, and separating biotinylated mRNA from unlabeled RNA. The methods provided herein may be used, for example, for gene discovery, drug screens and studies of the regulation of gene expression.

L'invention a pour objet des procédés d'isolement d'ARN messager. Ces procédés consistent à incorporer des analogues de NTPr biotinylés dans l'ARN messager cellulaire, et à séparer cet ARN messager biotinylé de l'ARN non marqué. Les procédés selon l'invention peuvent être appliqués pour découvrir des gènes, analyser des produits et étudier la régulation de l'expression génique.

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