Method for assaying creatine kinase isozyme activity and...

Details Method for assaying creatine kinase isozyme activity and... Method for assaying creatine kinase isozyme activity and...

G - Physics

01

N

G01N 33/573 (2006.01) C12Q 1/50 (2006.01)

Patent

CA 2324314

A highly convenient method for accurately assaying the activity of creatine kinase MB isozyme (CK-MB) wherein the activity of creatine kinase isozyme (mCK) localized in mitochondria is inhibited to thereby avoid the effects of mCK. This method is one for selectively inhibiting the enzymatic activity of creatine kinase (CK) isozyme by immune inhibition characterized in that creatine kinase M (CK-M) subunit and the enzyme reaction of mCK are selectively and immunologically eliminated and then the remaining CK activity is measured.

L'invention concerne une technique très appropriée pour analyser avec précision l'activité de l'isozyme MB de la créatine kinase (CK-MB), l'activité de l'isozyme de la créatine kinase (mCK) située dans les mitochondries étant inhibée, de façon à éviter les effets de mCK. Cette technique qui permet d'inhiber sélectivement l'activité enzymatique de l'isozyme de la créatine kinase (CK) par inhibition immune, est caractérisée en ce qu'une sous-unité de la créatine kinase M (CK-M) et la réaction enzymatique de mCK sont sélectivement et immunologiquement éliminées, et en ce que l'activité de la CK restante est mesurée.

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