Sink protein

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12P 13/08 (2006.01) C12P 13/12 (2006.01) C12P 13/22 (2006.01)

Patent

CA 2381556

The invention relates to the field of providing additional nutritional value to a (micro-)organism by equipping a cell or cells of said organism with means for producing desired levels of an amino acid, preferably a so-called essential amino acid. The invention provides a method for regulating the flux, concentration or biosynthesis of a desired amino acid in a cell comprising providing said cell with a nucleic acid encoding a proteinaceous substance comprising at least said amino acid, such a proteinaceous substance herein also called sink protein whereby sink stands for the capacity of said proteinaceous substance to function as sink or reservoir for the desired amino acid, thereby regulating or reducing the amount or concentration of available of free amino acid in said cell by binding it through transcription and translation processes to the sink protein encoded by the additional nucleic acid.

On dote un (micro)-organisme d'une valeur nutritive supplémentaire en fournissant à une cellule ou à des cellules de cet organisme des moyens de production de niveaux souhaités d'un acide aminé, de préférence un acide aminé considéré comme essentiel. L'invention concerne un procédé de régulation du flux, de la concentration ou de la biosynthèse d'un acide aminé voulu dans une cellule, le procédé consistant à doter cette cellule d'un acide nucléique codant pour une substance protéinique qui contient au moins l'acide aminé. Cette substance protéinique est aussi dénommée protéine puits, le terme puits renvoyant à la capacité de cette substance protéinique de fonctionner comme puits ou réservoir de l'acide aminé voulu, ce qui permet de réguler ou de réduire la quantité ou la concentration d'acides aminés libres disponibles dans la cellule en la liant par transcription et translation à la protéine puits codée par l'acide nucléique supplémentaire.

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