C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/14 (2006.01) C12N 15/09 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2471375
Using DNA microchips having 1265 types of rice ESTs bonded thereto, it is attempted to search for genes the expression of which is induced at the early stage of the elicitor treatment. Use is made therein of a chitin oligomer (N- acetylchitooligosaccharide) which is one of the major constituents of Pyricularia orizae cell wall. As a result, 6 types of novel elicitor- responsive ESTs are identified. Products of two genes (named CIGR1 gene and CIGR2 gene), from among these ESTs, have a motif characteristic to the GRAS family considered as transcriptional factors. Thus, it has been clarified for the first time that GRAS family members other than the gibberellin signal transduction regulator exist in rice.
L'invention concerne l'utilisation de micropuces a ADN comprenant 1265 types d'étiquettes exprimées de séquence (EST) de riz liées sur ces micropuces, dans le but de rechercher des gènes dont l'expression est induite à un étape précoce du traitement par l'éliciteur. On utilise un oligomère de chitine (N-acétylchitooligosaccharide) qui est l'un des constituants principaux de la paroi cellulaire de Pyricularia orizae. Il en résulte l'identification de 6 types d'EST sensible à l'éliciteur. Les produits de deux gènes (dénommés gènes CIGR1 et CIGR2), parmi ces EST, possèdent un motif caractéristique de la famille GRAS considérés en tant que facteurs de transcription. Ainsi, pour la première fois, il existe dans le riz des éléments de la famille GRAS autres que le régulateur de transduction du signal de la gibbérelline.
Day Robert B.
Minami Eiichi
Shibuya Naoto
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
National Institute Of Agrobiological Sciences
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