Process for serially culturing keratinocytes

Details Process for serially culturing keratinocytes Process for serially culturing keratinocytes

C - Chemistry, Metallurgy

12

N

195/46

C12N 5/00 (2006.01) C12N 1/10 (2006.01) C12N 5/06 (2006.01)

Patent

CA 1083987

PROCESS FOR SERIALLY CULTURING KERATINOCYTES ABSTRACT OF THE DISCLOSURE A method is disclosed for serially culturing keratino- cytes such as human epidermal cells and teratomal keratinocytes. These can be serially grown in culture to produce colonies and eventually stratified squamous epithelium by including in the culture fibroblast cells or medium harvested from fibroblast cultures, When fibroblast cells are used, they are treated to prevent their multiplication, and they are also introduced into the cultures at a controlled density.

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