Detection of non-viral organisms with srp rna

Details Detection of non-viral organisms with srp rna Detection of non-viral organisms with srp rna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2330732

The present invention provides rapid detection methods that detect virtually all non-viral organisms, from a broad to a narrow spectrum. More particularly, the present invention provides SRP RNA nucleic acid probes and methods of using such nucleic acid probes for the rapid, sensitive detection of non-viral organisms such as bacteria, fungi, and protozoa. Using the detection methods of the present invention, major non-viral groups such as bacterial, fungi, and protozoa, as well as specific species, can be identified in samples. In addition, kits for use in carrying out the methods of the present invention are provided.

La présente invention concerne des procédés qui permettent de détecter rapidement virtuellement tout organisme non-viral, quelle que soit l'étendue de son champ d'activité. Plus particulièrement, l'invention concerne des sondes d'acide nucléique d'ARN SRP et des procédés mettant en oeuvre lesdites sondes pour détecter rapidement et efficacement des organismes non-viraux, tels que des bactéries, des champignons ou des protozoaires. La mise en oeuvre des procédés de détection de l'invention permet d'identifier, dans des échantillons, des groupes non viraux principaux, tels que des bactéries, des champignons ou des protozoaires, ainsi que des espèces spécifiques. On décrit en outre des trousses servant à mettre en oeuvre les procédés de l'invention.

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