Monoclonal antibodies against cd30 lacking fucosyl residues

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 16/30 (2006.01)

Patent

CA 2598561

The invention pertains to anti-CD30 antibodies that lack fucosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to lyse CD30-expressing cell lines that are not lysed by the fucosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl residues, wherein the host cells are deficient for a fucosyl transferase. Methods of using the antibodies to inhibit the growth of CD30+ cells, such as tumor cells, are also provided.

La présente invention concerne des anticorps anti-CD30 qui sont dépourvus de résidus fucosyle. Les anticorps selon l'invention présentent une activité cytotoxique cellulaire dépendant des cellules qui est accrue, y compris la faculté de lyser des lignées cellulaires exprimant CD30 qui ne sont pas lysées par la forme fucosylée des anticorps. Cette invention se rapporte également à des cellules hôtes qui expriment les anticorps anti-CD30 dépourvus de résidus fucosyle, lesdites cellules étant déficientes en fucosyl transférase; et à des méthodes d'utilisation des anticorps pour inhiber la croissance de cellules CD30+, telles que les cellules tumorales.

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