Assay for antibodies to mycobacterium paratuberculosis

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/20 (2006.01) C07K 14/35 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2662323

A method of detecting an immune response to a paratuberculosis- specific antigen, comprising incubating a sample from a subject with the paratuberculosis-specific antigen and detecting the presence of an antibody in the sample as an indication of an immune response to the paratuberculosis- specific antigen. The antigen may be obtained from a novel M. parat.upsilon.berc.upsilon.losis strain JTC303. The antigen may be obtained from the JTC303 culture filtrate. Also provided are antibodies to the paratuberculosis- specific antigen, and a diagnostic kit for the detection of an immune response to a paratuberculosis-specific antigen in a mammal.

L'invention concerne un procédé de détection d'une réponse immunitaire à un antigène spécifique de la paratuberculose, comprenant l'incubation d'un échantillon issu d'un sujet avec l'antigène spécifique de la paratuberculose et la détection de la présence d'un anticorps dans l'échantillon comme indication d'une réponse immunitaire à l'antigène spécifique de la paratuberculose. L'antigène peut être obtenu à partir d'une nouvelle souche de bacille de Koch, JTC303. L'antigène peut être obtenu à partir du filtrat de culture de JTC303. L'invention concerne également des anticorps envers l'antigène spécifique de la paratuberculose et un kit de diagnostic pour la détection d'une réponse immunitaire à un antigène spécifique de la paratuberculose chez un mammifère.

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