Pre-cast electrophoresis slab gels with extended storage life

B - Operations – Transporting – 01 – D

Patent

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B01D 57/02 (2006.01) B01D 59/42 (2006.01) B01D 61/42 (2006.01) C02F 1/469 (2006.01) C07K 1/26 (2006.01) C08F 2/58 (2006.01) C25B 7/00 (2006.01) C25B 11/00 (2006.01) C25B 15/00 (2006.01) G01L 1/20 (2006.01)

Patent

CA 2511939

In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by including a nonionic amphiphilic polymer in the monomer solution from which the gel is formed and casting the gel with the polymer included. The nonionic amphiphilic polymer also prevents the resulting gel from sticking to the walls when the gel is to be removed from the cassette after electrophoresis.

Dans des cassettes de gels en plaques pré-coulés, la formation de voies dans lesquelles des protéines peuvent migrer entre le gel et les parois de la cassette, pour former des bandes sombres, est évitée par l'addition d'un polymère non ionique amphiphile dans la solution de monomère à partir de laquelle le gel est formé, et par coulage du gel avec le polymère inclus. Le polymère amphiphile non ionique empêche également que le gel obtenu ne colle aux parois lorsqu'il faut retirer le gel de la cassette après électrophorèse.

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