Detection of a therapeutic antibody in an experimental animal

Details Detection of a therapeutic antibody in an experimental animal Detection of a therapeutic antibody in an experimental animal

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2589054

The present invention relates to the field of therapeutic antibodies. It especially relates to the study of therapeutic antibodies in an experimental animal. The present invention discloses a method of detecting a therapeutic antibody in a sample obtained from an experimental animal comprising the steps of a) providing the sample to be analyzed, b) incubating said sample with an antibody binding to a therapeutic antibody and not binding to the immunoglobulin of said experimental animal, c) optionally incubating said sample with a reagent appropriate for the selective detection of total, active or antigen-bound therapeutic antibody, and d) correlating the complex formed in (b) or (c) to the concentration of said therapeutic antibody. A monoclonal antibody directed to a certain epitope that is present on all classes of human immunoglobulin of class G, but not on the immunoglobulin of any experimental animal except on the IgG of chimpanzees was used (MAB-M-R10Z8E9) .

Cette invention relève du domaine des anticorps thérapeutiques et plus spécifiquement de l'étude d'anticorps thérapeutiques chez un animal de laboratoire. Cette invention concerne un procédé de détection d'un anticorps thérapeutique dans un échantillon prélevé sur un animal de laboratoire, lequel procédé comprend les étapes consistant: (a) à prélever un échantillon à analyser; (b) à incuber cet échantillon avec un anticorps qui se lie à un anticorps thérapeutique et qui ne se lie pas à l'immunoglobuline dudit animal de laboratoire; (c) à éventuellement incuber cet échantillon avec un réactif permettant d'effectuer une détection sélective d'un anticorps thérapeutique lié à l'antigène, actif ou complet; et (d) à mettre le complexe formé à l'étape (b) ou (c) en corrélation avec la concentration de cet anticorps thérapeutique.

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