C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/42 (2006.01) A23K 1/16 (2006.01) A23L 1/302 (2006.01) A61K 8/67 (2006.01) A61K 8/99 (2006.01)
Patent
CA 2261286
The present invention provides a process for the preparation of a composition comprising natural vitamin B12, wherein said process comprises the steps of: a) obtaining microbial cells containing natural vitamin B12, b) causing opening of the microbial cells such that at least part of the soluble content of the cells comprising vitamin B12 is released in a liquid in which the cells are contained, c) separating the opened cells and the liquid comprising the vitamin B12, d) preparing a mixture of the vitamin B12 and at least a part of the opened cells, wherein the mixture has a vitamin B12 concentration on dry matter in excess of 0.1 % (w/w).
Cette invention concerne un procédé de préparation d'une composition qui comprend de la vitamine B12 naturelle. Ce procédé comprend les étapes suivantes: (a) obtenir des cellules microbiennes contenant de la vitamine B12 naturelle; (b) ouvrir ces cellules microbiennes de manière à ce qu'une partie au moins du contenu soluble des cellules contenant la vitamine B12, soit libérée dans un liquide contenant lesdites cellules; (c) séparer les cellules ouvertes du liquide contenant la vitamine B12; et enfin (d), préparer un mélange se composant de vitamine B12 et d'une partie au moins des cellules ouvertes. Ce mélange possède une teneur en vitamine B12 sur matière sèche qui est supérieure à 0,1 % (poids/poids).
Gist-Brocades B.v.
Osler Hoskin & Harcourt Llp
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