A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
A61K 49/00 (2006.01) C12P 19/12 (2006.01) C12Q 1/34 (2006.01)
Patent
CA 2209927
There is disclosed the use of O-.beta.-D-galactopyranosyl-D-xyloses, particularly 2-O-.beta.-D-galactopyranosyl-D-xylose, and 3-O-.beta.-D-galactopyranosyl-D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O-.beta.-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of .beta.-D-galactopyranoside in the presence of an enzyme .beta.-galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37°C; deactivating the .beta.-galactosidase by heating at 100°C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.
L'invention décrit l'utilisation de O- beta -D-galactopyranosyl-D-xyloses, en particulier 2-O- beta -D-galactopyranosyl-D-xylose et 3-O- beta -D-galactopyranosyl-D-xylose, pour la preparation de compositions des solutions destinées à l'évaluation de la lactase intestinale, et présentant une affinité à la lactase sensiblement améliorée. On décrit aussi un procédé pour l'obtention de O- beta -D-galactopyranosyl-D-xyloses, consistant à: faire réagir une D-xylose et un substrat de beta -D-galactopyranosyl en présence d'une enzyme beta -galactosidase, dans un milieu aqueux tamponné à un pH compris entre 5,0 et 9,0 et à une température entre 4 et 37 DEG C; désactiver la beta -galactosidase en chauffant à 100 DEG C lorsqu'on atteint le rendement maximum de formation de disaccharides déetecté par chromatographie en couche fine, et isolement des disaccharides formés par filtration en colonne garnie avec un diluant sélectionné entre l'eau ou eau/alcool.
Aragon Reyes Juan Jose
Canada Vicinay Francisco Javier
Fernandez-Mayoralas Alvarez Alfonso
Lopez Alvarez Rosa
Martin Lomas Manuel
Consejo Superior Investgaciones Cientificas
Consejo Superior Investigaciones Cientificas
Marks & Clerk
Universidad Autonoma
LandOfFree
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