A - Human Necessities – 01 – K
Patent
A - Human Necessities
01
K
A01K 67/00 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12N 15/00 (2006.01) C12N 15/87 (2006.01)
Patent
CA 2387488
The invention relates to a method of synchronizing a population of somatic cells in G1 for purposes of preparing the cells for nuclear transfer or nuclear transplantation by using mechanical shake-off and selection of mitotic doublet cells. This method may further comprise cooling of the cells or other means of synchronizing the cells in G1 phase for longer periods of time. The invention also relates to the use of a synchronized population of rapidly, dividing somatic cells obtained by these methods as a source of donor nuclei or chromatin for use in nuclear transfer or nuclear transplantation.
L'invention concerne un procédé de synchronisation d'une population de cellules somatiques dans G¿1? dans le but de préparer ces cellules à un transfert ou une transplantation nucléaires au moyen d'une technique de secousses mécaniques et d'une sélection de cellules mitotiques en doublet. Ce procédé peut, de plus, consister à refroidir les cellules ou en d'autres moyens de synchronisation de ces cellules en phase G¿1? pendant des durées plus longues. L'invention concerne également l'utilisation d'une population synchronisée de cellules somatiques à division rapide obtenues au moyen de ces procédés en tant que source de noyaux donneurs ou de chromatine afin de les utiliser en transfert ou transplantation nucléaires.
Jerry Joseph D.
Knott Jason G.
Poothappillai Kasinathan
Robl James M.
As Represented By Its Amherst Campus University Of Massachusetts A. Public Institution Of Higher Educ
Gowling Lafleur Henderson Llp
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