Chicken anemia virus vaccine from cell line

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/12 (2006.01) A61K 39/17 (2006.01) A61K 45/00 (2006.01) C12N 7/00 (2006.01) C12N 7/06 (2006.01) C12N 13/00 (2006.01)

Patent

CA 2558736

Provided is a chicken infectious anemia virus (CIAV) vaccine, comprising live CIAV passaged in MDCC-MSB-l (MSB-1) cells, wherein the vaccine does not cause Marek's Disease. Also provided is a CIAV vaccine comprising a CIA virus having the sequence of SEQ ID NO: 1. A method of making a CIAV vaccine is provided, comprising culturing CIAV in MSB-1 cells, and removing or killing any Marek's disease virus present in the CIAV-containing MSB-1 culture. Provided a method of immunizing a chicken against CIAV infection, comprising administering to the chicken an amount of the CIAV vaccine of the invention sufficient to induce an immune response to CIAV.

La présente invention concerne un vaccin contre l'anémie infectieuse du poulet (CIAV), lequel vaccin comprend un CIAV vivant introduit dans des cellules MDCC-MSB-l (MSB-1), ce vaccin ne provoquant pas la maladie de Marek. La présente invention concerne également un vaccin contre l'anémie infectieuse du poulet (CIAV) comprenant un virus CIA présentant la séquence SEQ ID NO: 1. L'invention concerne également un procédé permettant d'élaborer un vaccin contre l'anémie infectieuse du poulet (CIAV), lequel procédé consiste à cultiver un CIAV dans des cellules MSB-1, puis à retirer ou à tuer tout virus de la maladie de Marek se trouvant dans la culture de cellules MSB-1 contenant le virus CIAV. En outre, cette invention concerne un procédé permettant d'immuniser un poulet contre l'infection à CIAV, lequel procédé consiste à administrer au poulet une quantité suffisante dudit vaccin CIAV pour induire une réponse immunitaire au CIAV.

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