A dried and stabilized ready-to-use composition containing...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2761059

The present invention relates to a exsiccated or lyophilized composition comprising: a nucleic acid polymerization enzyme and cellobiose, in which the enzyme is stable for a period of time, even at a temperature of up to 55 °C. The composition of the invention can also comprise further reagents, such as salts, primers specific for a template DNA present in a sample, probes, etc., and possibly other stabilizing compounds. The invention relates to a method for preparing a exsiccated or lyophilized composition comprising a nucleic acid polymerization enzyme and cellobiose, possibly in containers, in which the enzyme is lyophilized and ready for use in molecular biology applications upon addition of the sample.

La présente invention concerne une composition déshydratée ou lyophilisée comprenant : une enzyme de polymérisation d'acide nucléique et du cellobiose, dans laquelle l'enzyme est stable pendant une certaine durée, même à une température allant jusqu'à 55 °C. La composition de l'invention peut comprendre en outre d'autres réactifs, tels que des sels, des amorces spécifiques pour une matrice d'ADN présente dans un échantillon, des sondes, etc., et éventuellement d'autres composés stabilisants. L'invention concerne un procédé pour préparer une composition déshydratée ou lyophilisée comprenant une enzyme de polymérisation d'acide nucléique et du cellobiose, éventuellement dans des récipients, dans lesquels l'enzyme est lyophilisée et prête pour utilisation dans des applications de biologie moléculaire après ajout de l'échantillon.

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