Method for producing double-stranded dna whose terminal...

Details Method for producing double-stranded dna whose terminal... Method for producing double-stranded dna whose terminal...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2286850

Disclosed is a method for producing double-stranded DNA comprising treating double-stranded DNA having a homopolymer part or parts at one or both ends with a restriction enzyme to partly or fully eliminate at least one of the homopolymer part or parts. The restriction enzyme is capable of cleaving double-stranded DNA at a cleavage site separate from a recognition site therefor. Disclosed is a method for determining a nucleotide sequence of double-stranded DNA utilizing one or both strands of the double-stranded DNA as a template, wherein the double-stranded DNA used as the template is double-stranded DNA prepared by the above method of the present invention. The present invention provides a method for producing double-stranded DNA a part or all of which homopolymer part, which may inhibit nucleotide sequence determination, is eliminated, and a method for determining a nucleotide sequence utilizing as a template the double-stranded DNA prepared by the above method of the present invention, whose homopolymer part is partially or fully eliminated.

Affiliated with

Also associated with

Rating

Method for producing double-stranded dna whose terminal... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing double-stranded dna whose terminal..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing double-stranded dna whose terminal... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1525681